5GQ9
Crystal structure of Thermus thermophilus Argonaute in complex with g1C siDNA and DNA target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97848 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.831, 116.503, 161.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.130 - 2.700 |
| R-factor | 0.1919 |
| Rwork | 0.189 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ncb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.542 | |
| Number of reflections | 58404 | |
| <I/σ(I)> | 21.4 | 2.45 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 308 | 2.8M sodium acetate, 0.1M Bis-tris Propane, pH 7.0 |
