5GOZ
Crystal structure of ZIKV NS5 Methyltransferase in complex with GTP and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2016-06-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 61 |
| Unit cell lengths | 123.790, 123.790, 120.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.520 - 2.049 |
| R-factor | 0.2368 |
| Rwork | 0.236 |
| R-free | 0.26280 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.044 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.049 |
| Number of reflections | 65414 |
| <I/σ(I)> | 14.3 |
| Completeness [%] | 99.8 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1M (NH4)2SO4, 10% PEG 8000, 0.1M SPG buffer |
