5GOM
Truncated mitofusin-1, transition-like state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.108, 45.982, 146.180 |
| Unit cell angles | 90.00, 92.23, 90.00 |
Refinement procedure
| Resolution | 48.690 - 2.802 |
| R-factor | 0.1926 |
| Rwork | 0.189 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.948 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.697 |
| High resolution limit [Å] | 2.802 |
| Number of reflections | 181542 |
| <I/σ(I)> | 20.55 |
| Completeness [%] | 99.1 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM MES (pH 6.0), 240mM NaCl, 36% pentaerythriol propoxylate (5/4 PO/OH) |
