5GOF

Truncated mitofusin-1, GTP-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	RAYONIX MX-225
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	70.579, 72.461, 95.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.105 - 1.604
R-factor	0.1811
Rwork	0.179
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.660
High resolution limit [Å]	1.600
Number of reflections	426491
<I/σ(I)>	25.54
Completeness [%]	89.4
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1mM ZnCl2, 100mM Tris-HCl (pH 8.0~9.0), 15~16% PEG 3350, 10~15% glycerol