5GO3
Crystal structure of a di-nucleotide cyclase Vibrio mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9789 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.745, 46.272, 121.791 |
Unit cell angles | 90.00, 99.82, 90.00 |
Refinement procedure
Resolution | 43.174 - 2.200 |
R-factor | 0.2314 |
Rwork | 0.228 |
R-free | 0.29210 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.202 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.092 | |
Number of reflections | 50934 | |
<I/σ(I)> | 18.6 | |
Completeness [%] | 98.0 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291 | 25% PEG 3350, 100 mM HEPES (pH 7.5), 200 mM magnesium chloride hexahydrate |