5GNS
Structures of human Mitofusin 1 provide insight into mitochondrial tethering
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.657, 74.671, 94.756 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.005 - 2.702 |
R-factor | 0.219 |
Rwork | 0.212 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.388 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 122150 |
<I/σ(I)> | 10.2 |
Completeness [%] | 99.1 |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 200mM sodium phosphate monobasic monohydrate, 20%(w/v) PEG3350 |