5GNS

Structures of human Mitofusin 1 provide insight into mitochondrial tethering

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	71.657, 74.671, 94.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.005 - 2.702
R-factor	0.219
Rwork	0.212
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.388
Data reduction software	HKL-2000
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	122150
<I/σ(I)>	10.2
Completeness [%]	99.1
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	200mM sodium phosphate monobasic monohydrate, 20%(w/v) PEG3350