5GN8

Structure of a 48-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	F 4 3 2
Unit cell lengths	236.120, 236.120, 236.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.741 - 2.805
R-factor	0.2153
Rwork	0.213
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fha
RMSD bond length	0.010
RMSD bond angle	0.895
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	13673
<I/σ(I)>	19.1
Completeness [%]	99.9
Redundancy	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		300	0.1 M imidazol-HCl at pH 7.5, 10% reagent alcohol (15%) and 0.2 M MgCl2