5GMQ
Structure of MERS-CoV RBD in complex with a fully human antibody MCA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 153.460, 153.460, 97.494 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.330 - 2.703 |
R-factor | 0.2414 |
Rwork | 0.239 |
R-free | 0.28460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5do2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.161 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.330 | 3.170 |
High resolution limit [Å] | 2.703 | 2.703 |
Number of reflections | 36296 | |
<I/σ(I)> | 9.9 | |
Completeness [%] | 99.3 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.06 M Citric acid, 0.04 M Bis-tris propane, 16% (w/v) PEG 3350 |