5GMG
Crystal structure of monkey TLR7 in complex with loxoribine and polyU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.277, 99.410, 112.096 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.600 |
| R-factor | 0.21719 |
| Rwork | 0.215 |
| R-free | 0.25833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gmf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.666 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Number of reflections | 58705 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 93.9 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, magnesium chloride, sodium citrate pH 5.0 |
