5GMF
Crystal structure of monkey TLR7 in complex with guanosine and polyU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 98.084, 111.335, 113.376 |
| Unit cell angles | 93.83, 94.34, 91.48 |
Refinement procedure
| Resolution | 50.010 - 2.500 |
| R-factor | 0.20961 |
| Rwork | 0.208 |
| R-free | 0.24324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w3j |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.737 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.921 | |
| Number of reflections | 153109 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 6000, calcium acetate, HEPES pH 7.0 |
