5GMF
Crystal structure of monkey TLR7 in complex with guanosine and polyU
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-23 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 98.084, 111.335, 113.376 |
Unit cell angles | 93.83, 94.34, 91.48 |
Refinement procedure
Resolution | 50.010 - 2.500 |
R-factor | 0.20961 |
Rwork | 0.208 |
R-free | 0.24324 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w3j |
RMSD bond length | 0.013 |
RMSD bond angle | 1.737 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.921 | |
Number of reflections | 153109 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 97.6 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 6000, calcium acetate, HEPES pH 7.0 |