5GMD
Crystal structure of Sulfolobus solfataricus diphosphomevalonate decarboxylase in complex with ATP-gamma-S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-11-05 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 151.238, 151.238, 104.416 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.500 - 1.500 |
R-factor | 0.16623 |
Rwork | 0.165 |
R-free | 0.19234 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4z7c |
RMSD bond length | 0.031 |
RMSD bond angle | 2.956 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.500 |
High resolution limit [Å] | 1.500 |
Number of reflections | 72082 |
<I/σ(I)> | 37.1 |
Completeness [%] | 99.2 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 293 | 0.1M sodium acetate, 1.5M ammonium sulfate |