5GL8
The crystal structure of Nattokinase fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2016-06-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.284, 73.959, 67.811 |
| Unit cell angles | 90.00, 97.47, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.23363 |
| Rwork | 0.231 |
| R-free | 0.29053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dww |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.935 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.770 |
| Number of reflections | 49947 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 99.6 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 288 | 0.1M HEPES ,0.8 M Potassium sodium tartrate tetrahydrate |
