5GL5
Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c): UDPG complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9202 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.660, 80.562, 154.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.290 - 1.900 |
| R-factor | 0.18712 |
| Rwork | 0.185 |
| R-free | 0.21936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.679 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0157) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.380 | 1.970 |
| High resolution limit [Å] | 1.903 | 1.903 |
| Rmerge | 0.164 | |
| Number of reflections | 65564 | |
| <I/σ(I)> | 18.04 | |
| Completeness [%] | 99.9 | |
| Redundancy | 14.5 | |
| CC(1/2) | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287.15 | PEG 3350, magnesium chloride, Tris pH8.5 |
