5GKV
Crystal Structure of a Novel Penicillin-Binding Protein (PBP) Homolog from Caulobacter crescentus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.919805 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.277, 67.308, 92.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.144 - 1.901 |
R-factor | 0.1726 |
Rwork | 0.170 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zyt |
RMSD bond length | 0.007 |
RMSD bond angle | 0.857 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.107 |
Number of reflections | 29140 |
<I/σ(I)> | 34.8 |
Completeness [%] | 100.0 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | 50mM potassium phosphate monobasic, 20%(w/v) polyethylene glycol 8,000 |