3ZYT

Structure Determination of EstA from Arthrobacter nitroguajacolicus Rue61a

Summary for 3ZYT

• Entry DOI: 10.2210/pdb3zyt/pdb
• Descriptor: ESTERASE A, ETHYL MERCURY ION (3 entities in total)
• Functional Keywords: hydrolase, enzymatic promiscuity, beta-lactamase
• Biological source: ARTHROBACTER NITROGUAJACOLICUS
• Total number of polymer chains: 1
• Total formula weight: 40312.69

Authors

Wagner, U.G.,Fetzner, S. (deposition date: 2011-08-25, release date: 2012-09-19, Last modification date: 2024-05-08)

Primary citation

Wagner, U.G.,Dimaio, F.,Kolkenbrock, S.,Fetzner, S.
Crystal Structure Analysis of Esta from Arthrobacter Sp. Rue61A--an Insight Into Catalytic Promiscuity.
FEBS Lett., 588:1154-, 2014

PubMed Abstract: In this article we analyze the reasons for catalytic promiscuity of a type VIII esterase with β-lactamase fold and the ability to cleave β-lactams. We compared the structure of this enzyme to those of an esterase of the same type without any lactamase ability, an esterase with moderate lactamase ability, and a class C β-lactamase with similar fold. Our results show that for these enzymes, the difference in the substrate specificity is sterically driven.

PubMed: 24613918
DOI: 10.1016/J.FEBSLET.2014.02.045

Experimental method

X-RAY DIFFRACTION (2.45 Å)