5GKR
Crystal structure of SLE patient-derived anti-DNA antibody in complex with oligonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-09 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 147.515, 147.515, 112.807 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.330 - 2.100 |
R-factor | 0.16359 |
Rwork | 0.163 |
R-free | 0.18512 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qhk 4y5y 4idj and 4LLW |
RMSD bond length | 0.007 |
RMSD bond angle | 1.295 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 53509 | |
<I/σ(I)> | 23.1 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.8 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl (pH 7.0), 0.2 M NaCl, 1 M Sodium citrate |