5GKK
Crystal structure of a homing endonuclease, I-TnaI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.54175 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.203, 113.886, 44.922 |
| Unit cell angles | 90.00, 110.41, 90.00 |
Refinement procedure
| Resolution | 39.490 - 2.001 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bp7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.275 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.719 |
| Number of reflections | 19699 | |
| <I/σ(I)> | 16.4 | 4.3 |
| Completeness [%] | 96.5 | 96 |
| Redundancy | 6.2 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 294 | 100 mM Hepes, 35% Jeffamine ED-2001 |
