5GKD
Structure of PL6 family alginate lyase AlyGC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.608, 142.840, 126.276 |
| Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
| Resolution | 34.450 - 2.194 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.19730 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.450 | 2.273 |
| High resolution limit [Å] | 2.194 | 2.194 |
| Rmerge | 0.124 | |
| Number of reflections | 193472 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG1500, 100 mM SPG pH=8.5 |
