5GJN

Crystal structure of Lysine decarboxylase from Selenomonas ruminantium in P43212 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 43 21 2
Unit cell lengths	105.966, 105.966, 73.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.2029
Rwork	0.196
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2plk
RMSD bond length	0.019
RMSD bond angle	1.921
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.073	0.058	0.304
Number of reflections	28705
<I/σ(I)>	15.6
Completeness [%]	99.0	88.9	100
Redundancy	11.1	9.7	10.8
CC(1/2)		0.995	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG 400, Sodium citrate, MgCl2