5GJM
Crystal structure of Lysine decarboxylase from Selenomonas ruminantium in C2 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.450, 70.794, 88.061 |
Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.910 |
R-factor | 0.18125 |
Rwork | 0.177 |
R-free | 0.23364 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2plj |
RMSD bond length | 0.012 |
RMSD bond angle | 1.704 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
Rmerge | 0.145 | 0.080 | 0.346 |
Number of reflections | 18678 | ||
<I/σ(I)> | 17.2 | ||
Completeness [%] | 95.2 | 93 | 94.2 |
Redundancy | 3.5 | 4.2 | 3.3 |
CC(1/2) | 0.989 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | Sodium citrate, Sodium cacodylate |