5GJF
Crystal structure of human TAK1/TAB1 fusion protein in complex with ligand 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-12-14 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.070, 132.960, 141.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.440 - 2.890 |
R-factor | 0.1994 |
Rwork | 0.198 |
R-free | 0.23360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2eva |
RMSD bond length | 0.007 |
RMSD bond angle | 1.178 |
Data scaling software | SCALA (3.3.15) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.441 | 48.441 | 2.970 |
High resolution limit [Å] | 2.890 | 12.920 | 2.890 |
Rmerge | 0.046 | 1.222 | |
Rmeas | 0.153 | ||
Rpim | 0.057 | ||
Total number of observations | 90311 | ||
Number of reflections | 12633 | ||
<I/σ(I)> | 12.7 | 11 | 0.6 |
Completeness [%] | 99.8 | 98 | 99.7 |
Redundancy | 7.1 | 5.4 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.94M sodium potassium phosphate, 20%(v/v) Glycerol as cryoprotectant |