5GJD
Crystal structure of human TAK1/TAB1 fusion protein in complex with ligand 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-31 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.960, 132.370, 142.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.700 - 2.790 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eva |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.216 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 2.860 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.109 | 1.173 |
| Number of reflections | 14035 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.997 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | 1.7M sodium potassium phosphate, 20%(v/v) Glycerol as cryoprotectant |
