5GIP

Crystal structure of box C/D RNP with 13 nt guide regions and 11 nt substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	97.916, 97.718, 128.793
Unit cell angles	108.98, 104.42, 89.86

Refinement procedure

Resolution	24.859 - 3.129
R-factor	0.1796
Rwork	0.178
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pla
RMSD bond length	0.013
RMSD bond angle	1.479
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.210
High resolution limit [Å]	3.120	3.130
Rmerge	0.087
Number of reflections	74336
<I/σ(I)>	22.5
Completeness [%]	97.3
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.7M DL-malic acid, 0.1M potassium sodium tartrate tetrahydrate