5GIC
Crystal structure of VDR in complex with DLAM-2P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2013-01-31 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.315, 42.444, 42.106 |
Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.352 |
R-factor | 0.2202 |
Rwork | 0.217 |
R-free | 0.25060 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.922 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.350 |
Number of reflections | 11424 |
<I/σ(I)> | 10.3 |
Completeness [%] | 99.0 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris-HCl, PEG 8000, NaCl |