5GIB
Succinic acid bound trypsin crystallized as dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.290, 58.260, 136.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.582 - 2.697 |
R-factor | 0.2348 |
Rwork | 0.229 |
R-free | 0.28560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i8h |
RMSD bond length | 0.002 |
RMSD bond angle | 0.603 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 68.280 |
High resolution limit [Å] | 2.670 |
Rmerge | 0.163 |
Number of reflections | 11637 |
<I/σ(I)> | 5.6 |
Completeness [%] | 92.6 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 15% PEG3350, 0.1 M Sodium acetate, 0.1 M Succinic acid |