5GHW
Crystal structure of broad neutralizing antibody 10E8 with long epitope bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.633, 81.231, 254.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.400 - 2.400 |
R-factor | 0.19366 |
Rwork | 0.192 |
R-free | 0.23041 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g6f |
RMSD bond length | 0.011 |
RMSD bond angle | 1.431 |
Data reduction software | MOSFLM (7.1.0) |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.400 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.046 | 0.202 |
Number of reflections | 24652 | |
<I/σ(I)> | 34 | 8 |
Completeness [%] | 90.0 | 56.2 |
Redundancy | 9.7 | 7 |
CC(1/2) | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.5 mM DPC 50% MPD 200 mM phosphate 100 mM TRIS |