5GHA
Sulfur Transferase TtuA in complex with Sulfur Carrier TtuB
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.28251, 1.28311, 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 54.139, 93.873, 97.532 |
Unit cell angles | 109.21, 104.57, 106.86 |
Refinement procedure
Resolution | 47.840 - 2.502 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.24650 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.678 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.840 |
High resolution limit [Å] | 2.500 |
Number of reflections | 105373 |
<I/σ(I)> | 16.24 |
Completeness [%] | 94.9 |
Redundancy | 1.97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M HEPES-NaOH, 0-20%(v/v) PEG6000 |