5GAP
Body region of the U4/U6.U5 tri-snRNP
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Energy Filter | GIF Quantum |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.6 |
Number of Particle | 473827 |
Software | RELION,CTFFIND,Coot,REFMAC |
Symmetry | C1 |
CTF correction | PHASE FLIPPING ONLY |