5G64
The complex between human IgE-Fc and two anti-IgE Fab fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-11 |
| Detector | PILATUS |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 76.640, 231.190, 247.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 115.595 - 3.715 |
| R-factor | 0.2614 |
| Rwork | 0.259 |
| R-free | 0.30920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2WQR AND UNPUBLISHED FAB STRUCTURE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.451 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.590 | 4.000 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.150 | |
| Number of reflections | 23989 | |
| <I/σ(I)> | 17.9 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 38 | 38.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4% PEG 8000 AND 0.03M SODIUM FLUORIDE. CRYSTALS WERE CRYOPROTECTED WITH 4M TMAO | |||
| 1 | 4% PEG 8000 AND 0.03M SODIUM FLUORIDE. CRYSTALS WERE CRYOPROTECTED WITH 4M TMAO |
