5G60
S.pneumoniae ABC-transporter substrate binding protein FusA in complex with nystose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.030, 129.740, 120.280 |
Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
Resolution | 119.540 - 1.990 |
R-factor | 0.17183 |
Rwork | 0.169 |
R-free | 0.22092 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g5y |
RMSD bond length | 0.016 |
RMSD bond angle | 1.742 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.370 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.125 | 0.833 |
Number of reflections | 144038 | |
<I/σ(I)> | 7.1 | 1.1 |
Completeness [%] | 97.3 | 77.2 |
Redundancy | 3.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 20 % PEG6000, 0.1M SODIUM ACETATE PH 5.0, 0.02 M CALCIUM CHLORIDE |