5G60
S.pneumoniae ABC-transporter substrate binding protein FusA in complex with nystose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.030, 129.740, 120.280 |
| Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
| Resolution | 119.540 - 1.990 |
| R-factor | 0.17183 |
| Rwork | 0.169 |
| R-free | 0.22092 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g5y |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.742 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.370 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.125 | 0.833 |
| Number of reflections | 144038 | |
| <I/σ(I)> | 7.1 | 1.1 |
| Completeness [%] | 97.3 | 77.2 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20 % PEG6000, 0.1M SODIUM ACETATE PH 5.0, 0.02 M CALCIUM CHLORIDE |
