5G5S
Structure of the Argonaute protein from Methanocaldcoccus janaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-16 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.477, 104.932, 115.077 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.120 - 2.290 |
R-factor | 0.2112 |
Rwork | 0.210 |
R-free | 0.23620 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Phasing software | CRANK |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.050 | 1.430 |
Number of reflections | 34953 | |
<I/σ(I)> | 18.8 | 1.19 |
Completeness [%] | 1.0 | 1 |
Redundancy | 7.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M TRI- SODIUM CITRATE, 8 % (W/V) POLYETHYLENE GLYCOL 4000 AND 10-12 % (V/V) ISOPROPANOL |