5G5G
Escherichia coli Periplasmic Aldehyde Oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.681, 78.342, 151.909 |
| Unit cell angles | 90.00, 99.69, 90.00 |
Refinement procedure
| Resolution | 48.320 - 1.700 |
| R-factor | 0.13988 |
| Rwork | 0.138 |
| R-free | 0.16745 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1RM6 1t3q 1ffu 1ffv |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.046 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.110 | 0.860 |
| Number of reflections | 137342 | |
| <I/σ(I)> | 9.69 | 1.9 |
| Completeness [%] | 98.5 | 99 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PEG 3350, AMMONIUM IODIDE, pH 7.5 |
