5G5A
Glutathione transferase U25 from Arabidopsis thaliana in complex with glutathione disulfide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2016-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.830, 107.668, 108.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.540 - 1.950 |
| R-factor | 0.20521 |
| Rwork | 0.205 |
| R-free | 0.21736 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4top |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.935 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.540 | 1.990 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.540 |
| Number of reflections | 75638 | |
| <I/σ(I)> | 12.7 | 3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.2 M AMMONIUM ACETATE, 0.1 M BIS-TRIS PROPANE PH 5.5 AND 23 % (W/V) PEG 3350; 2 MM GSH |
