5G4P
Crystal structure of human hippocalcin at 2.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 |
| Unit cell lengths | 50.970, 50.970, 284.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.430 - 2.420 |
| R-factor | 0.22766 |
| Rwork | 0.225 |
| R-free | 0.27685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aeq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.562 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.560 | 2.590 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.040 | 0.620 |
| Number of reflections | 15734 | |
| <I/σ(I)> | 11.07 | 1.8 |
| Completeness [%] | 98.8 | 99.9 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.5, 18% V/V 2-PROPANOL, 16% W/V PEG 4000 |
