5G3T
The structure of the L-tryptophan oxidase VioA from Chromobacterium violaceum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.090, 89.160, 144.430 |
Unit cell angles | 90.00, 92.66, 90.00 |
Refinement procedure
Resolution | 19.992 - 1.800 |
R-factor | 0.1585 |
Rwork | 0.157 |
R-free | 0.19220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 0.935 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.980 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.100 | 0.950 |
Number of reflections | 157109 | |
<I/σ(I)> | 10.91 | 2 |
Completeness [%] | 98.0 | 97.3 |
Redundancy | 6.9 | 6.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 277 | 0.08 M TRIS PH 8.5, 24 %(W/V) PEG 4000, 0.16 M MGCL2, 20 %(V/V) GLYCEROL AT 277 K |