5G2E

Structure of the Nap1 H2A H2B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-19
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	111.470, 211.130, 126.570
Unit cell angles	90.00, 99.72, 90.00

Refinement procedure

Resolution	50.000 - 6.700
R-factor	0.2626
Rwork	0.263
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z2r
RMSD bond length	0.003
RMSD bond angle	0.753
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	7.000
High resolution limit [Å]	6.700	6.700
Rmerge	0.100	0.710
Number of reflections	10467
<I/σ(I)>	11.8	2.1
Completeness [%]	99.4	99.7
Redundancy	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				10-15% W/V PEG3350; 200 MM LICL
1				10-15% W/V PEG3350; 200 MM LICL
1				10-15% W/V PEG3350; 200 MM LICL