5G1X
Crystal structure of Aurora-A kinase in complex with N-Myc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-20 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 86.520, 86.520, 92.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 74.929 - 1.720 |
R-factor | 0.1771 |
Rwork | 0.176 |
R-free | 0.19510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ceg |
RMSD bond length | 0.005 |
RMSD bond angle | 1.043 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.930 | 1.760 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.060 | 0.740 |
Number of reflections | 42854 | |
<I/σ(I)> | 19.8 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.7 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 100 MM BICINE PH 9.0, 20% PEG 6000 |