5G1S
Open conformation of Francisella tularensis ClpP at 1.7 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 98.226, 127.924, 353.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 176.920 - 1.700 |
| R-factor | 0.20608 |
| Rwork | 0.204 |
| R-free | 0.24195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | OPEN CONFORMATION OF FRANCISELLA TULARENSIS CLPP AT 1.9 A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.420 |
| Number of reflections | 460688 | |
| <I/σ(I)> | 9.3 | 1.4 |
| Completeness [%] | 94.8 | 70.2 |
| Redundancy | 5.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | RATIO 1:1, 293K, HANGING DROP. RESERVOIR CONDITION: 0.2 M NACL, 30% (V/V) MPD AND 0.1 M SODIUM ACETATE PH 4.6 |
