5G1D

The complex structure of syntenin-1 PDZ domain with c-terminal extension

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	77
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	67.525, 88.529, 90.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.810
R-factor	0.19624
Rwork	0.194
R-free	0.23493
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.027
RMSD bond angle	2.269
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.070	0.290
Number of reflections	13799
<I/σ(I)>	24	4.36
Completeness [%]	97.6	93.9
Redundancy	8.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2 M LITHIUM SULFATE MONOHYDRATE, 0.1 M TRIS HYDROCHLORIDE (PH 8.5), 30% (W/V) POLYETHYLENE GLYCOL 4000