5G1B
Bordetella Alcaligenes HDAH native
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-07 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 100.660, 100.660, 175.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.560 - 1.700 |
R-factor | 0.15371 |
Rwork | 0.152 |
R-free | 0.19147 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g0x |
RMSD bond length | 0.010 |
RMSD bond angle | 1.508 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.600 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.130 | 0.850 |
Number of reflections | 99299 | |
<I/σ(I)> | 8.4 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.25 | pH 5.25 |