5G1A
Bordetella Alcaligenes HDAH bound to PFSAHA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 101.440, 101.440, 175.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.960 - 1.420 |
| R-factor | 0.14481 |
| Rwork | 0.143 |
| R-free | 0.17005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g0x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.520 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.700 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.080 | 0.870 |
| Number of reflections | 168945 | |
| <I/σ(I)> | 11.8 | 2.3 |
| Completeness [%] | 97.4 | 95.4 |
| Redundancy | 6.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.25 | pH 5.25 |
