5G0G
Crystal structure of Danio rerio HDAC6 CD1 in complex with trichostatin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.470, 93.010, 118.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.505 - 1.499 |
| R-factor | 0.1404 |
| Rwork | 0.139 |
| R-free | 0.16960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOMOLOGY MODEL |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.791 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 1.220 |
| Number of reflections | 51661 | |
| <I/σ(I)> | 20.6 | 1.3 |
| Completeness [%] | 98.1 | 91.8 |
| Redundancy | 5.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 23% PEG 3350, 0.2M MGCL2, 0.1M HEPES PH 7.0, SB ADDITIVE A7 |
