5FY1
Crystal structure of JmjC domain of human histone demethylase UTY in complex with N08619b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-23 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.710, 110.080, 118.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.193 - 1.780 |
R-factor | 0.2031 |
Rwork | 0.201 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zli |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | DIMPLE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.190 | 1.830 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.080 | 1.500 |
Number of reflections | 114896 | |
<I/σ(I)> | 14.7 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 0.1M HEPES PH 7.2 -- 25% PEG3350 |