5FXY
Structure of the human RBBP4:MTA1(464-546) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-24 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.294, 150.072, 95.591 |
| Unit cell angles | 90.00, 94.54, 90.00 |
Refinement procedure
| Resolution | 95.290 - 3.200 |
| R-factor | 0.25114 |
| Rwork | 0.249 |
| R-free | 0.29084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pc0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.053 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.300 | 3.340 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.160 | 0.800 |
| Number of reflections | 35054 | |
| <I/σ(I)> | 5.3 | 2 |
| Completeness [%] | 97.7 | 98 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.21 M AMMONIUM CITRATE AND 19% PEG 3350, pH 7.5 |
