4PC0

Structure of the human RbAp48-MTA1(670-711) complex

Summary for 4PC0

• Entry DOI: 10.2210/pdb4pc0/pdb
• Related: 4PBY 4PBZ
• Descriptor: Histone-binding protein RBBP4, Metastasis-associated protein MTA1, GLYCEROL, ... (5 entities in total)
• Functional Keywords: cell cycle, mta1-nurd subcomplex
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus: Q09028; Isoform Short: Cytoplasm. Isoform Long: Nucleus: Q13330
• Total number of polymer chains: 4
• Total formula weight: 104896.11

Authors

Alqarni, S.S.M.,Silva, A.P.G.,Mackay, J.P.,Laue, E.D. (deposition date: 2014-04-14, release date: 2014-06-11, Last modification date: 2023-12-20)

Primary citation

Alqarni, S.S.,Murthy, A.,Zhang, W.,Przewloka, M.R.,Silva, A.P.,Watson, A.A.,Lejon, S.,Pei, X.Y.,Smits, A.H.,Kloet, S.L.,Wang, H.,Shepherd, N.E.,Stokes, P.H.,Blobel, G.A.,Vermeulen, M.,Glover, D.M.,Mackay, J.P.,Laue, E.D.
Insight into the architecture of the NuRD complex: Structure of the RbAp48-MTA1 sub-complex.
J.Biol.Chem., 289:21844-, 2014

PubMed Abstract: The nucleosome remodeling and deacetylase (NuRD) complex is a widely conserved transcriptional co-regulator that harbors both nucleosome remodeling and histone deacetylase activities. It plays a critical role in the early stages of ES cell differentiation and the reprogramming of somatic to induced pluripotent stem cells. Abnormalities in several NuRD proteins are associated with cancer and aging. We have investigated the architecture of NuRD by determining the structure of a subcomplex comprising RbAp48 and MTA1. Surprisingly, RbAp48 recognizes MTA1 using the same site that it uses to bind histone H4, showing that assembly into NuRD modulates RbAp46/48 interactions with histones. Taken together with other results, our data show that the MTA proteins act as scaffolds for NuRD complex assembly. We further show that the RbAp48-MTA1 interaction is essential for the in vivo integration of RbAp46/48 into the NuRD complex.

PubMed: 24920672
DOI: 10.1074/jbc.M114.558940

Experimental method

X-RAY DIFFRACTION (2.5 Å)