5FWP
Atomic cryoEM structure of Hsp90-Cdc37-Cdk4 complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Single blot from 4 to 6 seconds, at 20C |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.2 |
| Number of Particle | 114683 |
| Software | Rosetta,UCSF Chimera,RELION |
| Symmetry | C1 |
