5FV4
Pig liver esterase 5 (PLE5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.860, 188.020, 109.500 |
| Unit cell angles | 90.00, 96.44, 90.00 |
Refinement procedure
| Resolution | 71.140 - 2.400 |
| R-factor | 0.18043 |
| Rwork | 0.179 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mx9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.562 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.140 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.770 |
| Number of reflections | 144950 | |
| <I/σ(I)> | 12.8 | 2.02 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | 100 MM HEPES PH 7.5, 18% PEG 4000 AT 295 K |
