5FT4
Crystal structure of the cysteine desulfurase CsdA from Escherichia coli at 1.996 Angstroem resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.095, 102.561, 71.258 |
| Unit cell angles | 90.00, 114.45, 90.00 |
Refinement procedure
| Resolution | 38.518 - 1.996 |
| R-factor | 0.1405 |
| Rwork | 0.138 |
| R-free | 0.19140 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.329 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.740 |
| Number of reflections | 191504 | |
| <I/σ(I)> | 11.18 | 1.85 |
| Completeness [%] | 99.3 | 96.1 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
