5FSU
Crystal structure of Trypanosoma brucei macrodomain (crystal form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-03 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 77.140, 77.140, 427.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.750 - 1.950 |
| R-factor | 0.19042 |
| Rwork | 0.189 |
| R-free | 0.21912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iqy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.090 | |
| Number of reflections | 56483 | |
| <I/σ(I)> | 16.9 | 1.8 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 15.1 | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M SODIUM SULFATE, 0.1 M BIS-TRIS PROPANE PH 7.5, 20 % W/V PEG 3350 |
