5FST
Structure of lysozyme prepared by the 'soak-and-freeze' method under 100 bar of krypton pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.001, 79.001, 37.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.860 - 1.200 |
| R-factor | 0.11 |
| Rwork | 0.109 |
| R-free | 0.13445 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lz8 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.042 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.860 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | 0.540 |
| Number of reflections | 70048 | |
| <I/σ(I)> | 24.7 | 4.65 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 13.8 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 10% (W/V) NACL, 0.1M NA ACETATE PH 4.8 25% (V/V) ETH-GLYCOL |
